By Tzvi Tzfira, Vitaly Citovsky

ISBN-10: 0387722890

ISBN-13: 9780387722894

ISBN-10: 0387722904

ISBN-13: 9780387722900

ISBN-10: 2007942364

ISBN-13: 9782007942361

Agrobacterium is the single mobile organism on the earth that's certainly able to shifting genetic fabric among the kingdoms of lifestyles, from prokaryotes to eukaryotes. reports have exposed a wealth of data at the means of Agrobacterium-mediated genetic transformation and at the bacterial and host phone components focused on the an infection. Agrobacterium has been proven to genetically rework, less than laboratory stipulations lots of plant species and diverse non-plant organisms, indicating the actually simple nature of the transformation technique. it truly is consequently now not mind-blowing that Agrobacterium and the genetic transformation itself have additionally develop into the point of interest of various moral and criminal debates.

Agrobacterium is a complete booklet on Agrobacterium examine, together with its heritage, program, uncomplicated biology discoveries, and results on human society. even if the e-book mostly makes a speciality of supplying a close overview of just about all molecular occasions of the genetic transformation strategy, it additionally presents assurance of moral and felony concerns proper to using Agrobacterium as a genetic transformation desktop. the result's an all-inclusive textual content which readers together with scientists and scholars desirous about plant genetic engineering will locate valuable as a reference resource for all significant points of the Agrobacterium-mediated genetic transformation of plant and non-plant organisms.

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Extra info for Agrobacterium: from biology to biotechnology

Example text

The tar region consists of the ttuA-E genes, orfX, orfY and a second ttuC copy. , 1996). The tar regions are thus found in different plasmid contexts and are also present in other bacteria, like Pseudomonas putida (Tipton and Beecher, 1994). Two tumor-inducing plasmids, pTiAB3 and pTiAT6, also carry tar genes. pTiAB3 and pTiAT6 belong to the IncRh1/2 group, pTrS4 to IncRh2, pTrAT6, pTrAB4 and pTrRr4 to IncRh4, while pTrTm4, pTrAB3 and pTrNW221 belong to other, as yet unidentified Inc groups (Szegedi and Otten, 1998).

Some authors have presented evidence that secondary root formation induced by A. rhizogenes can have beneficial effects on infected plants. A. , 1988). Therefore natural or artificial infection using this ‘pathogen’ has considerable potential in agriculture, especially in arid regions. This approach could be improved by stable introduction of the A. , 1999). The host range of Agrobacterium is determined by bacterial and plant factors. The former include bacterial virulence genes and T-DNA oncogenes, the latter plant genes required for transformation and tumor formation (see other chapters in this volume).

2000). Whether nematodes are able to carry the bacteria from one plant to another is still unknown. e. by growing non-hosts plants and by eliminating nematodes as far as possible. , 2001; Pulawska and Sobiczewski, 2005) is also essential. 3 Propagating material as a source of infection Pathogenic agrobacteria invade the whole plant from roots and tumors via the vessels; healthy parts of the plant can become latently infected without appearance of symptoms. g. , 1991). Since in the case of grapevine the infected propagating material is the primary source in the spreading of the pathogen it is of basic importance to select or produce pathogen-free Agrobacterium: A Disease-Causing Bacterium 17 stocks to reduce economic loss caused by crown gall.

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Agrobacterium: from biology to biotechnology by Tzvi Tzfira, Vitaly Citovsky

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